Satellite Meeting 2 (Windsor Suite)
Infrared Microspectroscopy
Organised by Dr. Mark Tobin, Daresbury
Abstract
The new infrared microspectroscopy beamline at the SRS has been operating as a user facility for over a year, and as expected, has shown a significant improvement in performance when compared with its predecessor. This meeting will provide the first opportunity for the developing user community to come together to hear about the work being undertaken on the beamline and to discuss potential future developments of the beamline. It will also be an ideal opportunity for interested potential users to discuss ideas with other users, and SRS staff.
Tuesday 13th (afternoon)
- 15:00 Ehsan Gazi (Paterson Institute, Manchester)
- Applications of FTIR Microspectroscopy in Studies of Prostate Cancer Tissue and Cell Lines
- 15:35 Dr. Fiona Lyng (Dublin Institute of Technology, Dublin)
- A study examining the effects of tissue processing on human tissue sections using vibrational spectroscopy
- 16:10-16:25 Coffee Break
- 16:25 Mark Tobin (Daresbury Laboratory)
- Update on current performance of Beamline 11 at the SRS
- 16:50 Dr. David A.Moss (ANKA)
- Practical tests of a focal plane array detector microscope at the ANKA-IR beamline
- 17:25 Azzedine Hammiche (Lancaster University )
- Photothermal Microspectroscopy using Synchrotron Radiation
Wednesday 14th (morning)
- 09:00 Nati Salvado (Universitat Politècnica de Catalunya, Barcelona )
- Advantages of the Use of SR-FT-IR Microspectroscopy: Applications to Cultural Heritage
- 09:40 Josep Sulé-Suso (North Staffordshire Hospital )
- Effects of gemcitabine
to in
vitro growing
lung cancer cells measured with Fourier
Transform Infrared microspectroscopy
Sulé-Suso J.
Staffordshire Oncology Centre, University Hospital of North Staffordshire , Stoke on Trent, U. K. and Institute for Science and Technology in Medicine, Keele University , Stoke on Trent U. K.ABSTRACT:
Background : Chemotherapy has become one of the main treatments for patients with lung cancer. However, it would be ideal to have a tool that could allow clinicians to determine what would be the best combination of chemotherapeutic drugs for each individual patient. To this purpose, we studied whether Fourier Transform Infrared (FTIR) microspectroscopy could become such a tool.Materials and Methods : Two lung cancer cell lines (CALU-1, and SKMES) were used in this study. Spectra were obtained by seeding 5 x 104 lung cancer cells in 100 µL of complete media on aluminium coated slides. After 1 hour incubation at 37°C and 5% CO2 , gemcitabine at different doses was added to cell cultures. After an overnight incubation, samples were washed three times with 0.9% NaCl. Samples were then kept at -80° before obtaining their micro-FTIR spectra with a Nicolet Centaurus IR microscope. Spectra were recorded in reflectance at a resolution of 4 cm-1 and with 64 co-additions. Synchrotron based micro-FTIR spectra were also obtained with a Thermo Nicolet Continuum FTIR microscope on beamline 11.1 at the Synchrotron Radiation Source, Daresbury Laboratory, U.K. Single cell spectra were recorded with a 10 µm aperture at a resolution of 4 cm-1 and with 128 co-additions.
Results : Cell survival decreased proportionally to the addition of gemcitabine. Furthermore, lung cancer cells became rounded and pyknotic following the addition of this drug. This correlated with changes in their micro-FTIR spectra. In fact, the amide I peak at 1645 cm-1 (C=O stretching vibrations) shifted to lower wavenumbers following the addition of gemcitabine to in vitro growing lung cancer cells. This shift has been associated with cell death.
Conclusion : microscopic FTIR spectroscopy could have a potential as a tool to assess tumour response to chemotherapy.
Supported by CCLRC Daresbury Laboratory and the Franco-British Partnership Programme.
- 10:20 Fariba Bahrami (Daresbury Laboratory)
- Future directions for Beamline 11 at the SRS - discussion with users
