BIOXHIT Section 3
Dissemination Meeting
DARESBURY LABORATORY, Daresbury, Warrington,
Cheshire. WA4 4AD
Wednesday 23rd and Thursday 24th November 2005
BIOXHIT Section 3 Dissemination Meeting - Tower Seminar Room
PROGRAMME
During the meeting we hope to be able to demonstrate a complete pipeline from submission of crystals to the synchrotron (with accompanying information) to return of processed data, linking to structure solution. Demonstrations of these steps, using a station at the SRS, will take place during the first day with the various stages being monitored remotely (see items in italics).
Day 1 - Wednesday 23rd November
08:30 - Coach departs from Holiday
Inn, Runcorn to Daresbury Laboratory
- Registration from 09:00 - 09:15
- 09:15 - Introduction
- Colin Nave
- 09:30 - Pipelining1 Demonstration of shipping crystals to synchrotron
- John Cowan
3.1 Tracking Crystalline Samples
This covers procedures for tracking crystals (e.g. using bar codes) sent to the synchrotron and the necessary accompanying information.
- 09:50 - Introduction
- Takashi Tomizake
- 10:00 - Experience with RFIDs for sample tracking
- Takashi Tomizake
- 10:25 - ISpyB, a LIMS for handling information about crystals sent to the synchrotron
- Ludovic Launer
- 10:50 Coffee
3.2 Automated Crystal Mounting and Centring
This covers procedures for centring the crystal in the x-ray beam using optical, UV or x-ray methods.
- 11:10 - Introduction
- Colin Nave
- 11:20 - Automatic Crystal Centring with XREC
- Babu Pothineni (EMBL Hamburg )
- 11:45 - Automatic Crystal centring using C3D
- Bernard Lavault (EMBL Grenoble)
- 12:10 - Automatic loop centring / fluorescence based crystal centring
- Alke Meents / Anuschka Pauluhn (SLS)
- 12:35 - Lunch
3.2 (continued)
- 13:25 Implementation of automatic crystal-centering on station 3 of Cassiopeia
- Krister Larsson
- 13:50 - Implementation of automatic crystal-centering on station 14.2 Daresbury
- Mylrajan Muthusamy
- 14:15 - Pipelining 2, Demonstration of DNA collecting data on the beamline
3.3 Crystal Characterisation
This covers initial characterisation of the crystal while still in the crystallisation well, together with improved detectors for characterising the x-ray fluorescence prior to anomalous dispersion data collection.
3.4 Strategic Preparation of diffraction experiment
This covers prediction of radiation damage affects, steps to minimise it, planning diffraction experiments and use of low x-ray energies.
- 16:00 - Introduction
- Andrea Schmidt
- 16:10 - Radiation damage for microcrystals
- John Cowan (SRS)
- 16:35 - Use of scavengers to reduce radiation damage
- Brice Kauffmann (EMBL Hamburg)
- 17:00 - Laser processing of crystals at cryo-temperatures
- Takashi Tomizake (SLS)
- 17:20 - Close
17:45
- Coach departs from Daresbury Laboratory to Holiday Inn, Runcorn
19:30
- Conference Dinner, Holiday Inn, Runcorn
Day 2 - Thursday 24th November
08:30 - Coach departs from Holiday Inn, Runcorn
to Daresbury Laboratory
3.4 (continued)
- 9:15 Radiation damage modelling (in BEST)
- Sasha Popov (or Gleb Bourenkov) (EMBL Hamburg)
- 9:40 Use of on line microspectrometer to monitor crystal
- John McGeehan (EMBL Grenoble)
3.5 Procedure for carrying out diffraction experiment
This covers automation of data collection (e.g. the DNA collaboration), development of kappa goiniometers and remote operation.
- 10:05 Introduction
- Alexander Popov (EMBL Hamburg )
- 10:15 Crystal screening and crystal ranking within DNA
- Romeu Pieritz (ESRF)
- 10:40 Break (Tea/Coffee)
- 11:05 Update on the SLS kappa development and related subjects
- Clemens Schulze-Briese (SLS)
- 11:25 STAC - STrategy for Aligned Crystals, an Object Oriented software for automated kappa collections
- Sandor Brockhauser (EMBL Grenoble)
- 11:50 Technology used by e-HTPX for remote operation
- David Meredith (Daresbury)
- 12:15 Pipelining 3, Demonstration of remote processing of data
- Graeme Winter
- 12:40 Lunch
- 13:40 General Discussion
- 14:45 Close
- 14:45 Meeting of section 3 workpackage coordinators
- 14:45 Tour of SRS
For questions regarding arrangements for the workshop (travel, accommodation
etc.) please contact the meeting administrator.
Alison Hannah
Tel: +44 (0)1925-603363
email: a.m.hannah@dl.ac.uk
