Daresbury Laboratory
Keckwick Lane, Daresbury
Warrington, Cheshire
WA4 4AD (UK)  

m.l.martin-fernandez@dl.ac.uk
(44) 1925 603568
(44) 1925 603124

Profile

My research is focused on the development and application of time-resolved microfluorimetry, confocal fluorescence lifetime imaging and single molecule detection techniques to the understanding of three crucial biological phenomena:

1.- Cell signalling by the epidermal growth factor receptor (funded by the BBSRC).

2.- Virus-host cell interactions during the initial stages of adenovirus (Ad) infection (in collaboration with Drs. George Santis and Brian Sutton, KCL), funded by the BBSRC.

3.- In vivo identification of interacting partners of S100A4 (p9Ka) (incollaboration with Drs. Roger Barraclough and Prof. Phillip Rutland, Liverpool University), funded by the NWCRF.

1. Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy. Webb SED, Needham SR, Roberts SK, Martin-Fernandez ML. (2006). Optics Letters 31 (14), 2157-2159.

2. Interaction of metastasis-inducing S100A4 protein in vivo by fluorescence lifetime imaging microscopy. Zhang S, Wang GZ, Fernig DG, Rudland PS, Webb SED, Barraclough R, Martin-Fernandez M. (2005). European Biophysics Journal with Biophysics Letters 34 (1) 19-27.

3. Mutually antagonistic actions of S100A4 and S100A1 on normal and metastatic phenotypes. Wang GZ, Zhang S, Fernig DG, Martin-Fernandez M, Rudland PS, Barraclough R. (2005). Oncogene 24 (8), 1445-1454.

4. Adenovirus Type 5 Entry and Disassembly Followed in Living Cells by FRET, Fluorescence Anisotropy, and FLIM. M.L. Martin-Fernandez, S.V. Longshaw, I. Kirby, G. Santis, M.J. Tobin, D.T. Clarke, and G.R. Jones (2004).  Biophys. J. 87:1316-1327.

5. Binding of Metastasis-associated S100A4 Protein to Non-muscle Myosin Heavy Chain A in vivo Visualized by Fluorescence Resonance Energy Transfer Microscopy. S. Zhang, G. Wang, D.G. Fernig, P.S. Rudland, S.E.D. Webb, R. Barraclough, and M.L. Martin Fernandez. (2004).  Eur. Biophys. J. Published online: 3 August 2004

6. Heterodimerisation and heterodimeric interfaces of S100A1 and S100P. G. Wang, S. Zhang, D.G. Fernig, D. Spiller, M.L. Martin Fernandez, H. Zhang, Y. Ding, Z. Rao, P.S. Rudland, and R. Barraclough. (2004).  Biochem. J. 382: 375-383 Part 1.

7. High resolution LAPS using amorphous silicon as the semiconductor material. Moritz W, Yoshinobu T, Finger F, F, Krause S, Martin-Fernandez M, Schoning MJ. (2004).  Sensor Actuat B-Chem 103 (1-2): 436-441.

   M.L. Martin-Fernandez, M.J. Tobin, S.V. Jones, D.T. Clarke, and G.R. Jones(2002). Preformed Oligomeric EGF Receptors Undergo an Ectodomain Structure Change during Signalling. Biophys. J. 82:2415-2427.

8. N.J. Gay, M. Symmons, M. Martin-Fernandez, G. Jones. (2001). Structure and Stabilisation of Self-assembling Peptide Filaments. In Self-assembling Peptide Systems in Biology, Medicine and Engineering. Edt A. Aggeli, N. Boden and S. Zhang. Kluwer Academic Publishers, pp 113-125.

9. Martin-Fernandez, M.L., Clarke, D.T., Tobin, M.J., and Jones, G.R. (2000). Real-time studies of the interactions between epidermal growth factor and its receptor during endocytic trafficking. Cell. Mol. Biol. 46, 1103-1112.

10. Tobin, M.J., Martin-Fernandez, M., and Jones, G.R. (1998). A facility for confocal imaging and microvolume fluorescence lifetime spectroscopy at the SRS. SRN 11(4): 24-30.

11. Martin-Fernandez, M.L., Tobin, M.J., Clarke, D.T., Gregory, C.M., and Jones, G.R. (1998). Sub-nanosecond polarised microfluorimetry in the time domain: An instrument for studying receptor trafficking in live cells. Rev. Sci. Instrum. 69, 540-543.

12. Martin-Fernandez, M.L., Tobin, M.J., Clarke, D.T., Gregory, C.M., and Jones, G.R. (1996). A high sensitivity time-resolved microfluorimeter for real-time cell biology. Rev. Sci. Instrum. 67, 3716-3721.

1. Martin-Fernandez, M.L., Tobin, M.J. Barraclough, B.R., Smith, J.A., and Derbyshire, G. Probing conformational dynamics in signalling EGF receptors via multidimensional single-molecule fluorescence microscopy. Biomolecular Science Committee, BBSRC. (2003-2005).
2. Barraclough, B.R., Rudland, P.S., and Martin-Fernandez, M.L. Interacting Partners of S100A4 (p9Ka) in vivo. NWCRF (2002-2004).